![]() ![]() These advances keep this tool more relevant than ever. Furthermore, new tools make it possible for scientists to use the bulk of Western-blotting knowledge that is available in the literature. Most important, new techniques make it possible to apply Western blotting in simpler ways that provide more accuracy than ever. In particular, this molecular technique can reveal how disease emerges, how it spreads, and ways to defeat it. Scientists around the world continue to use Western blotting in basic and applied research, such as pharmaceutical studies. Also, there’s really nothing “western” about it the name just emerged from a twist on the name Southern blotting, which is used to detect DNA.īy subscribing, you agree to receive email related to Lab Manager content and products. In brief, Western blotting separates proteins by size and shape with an electric field applied to a gel, and then targeted proteins are stained with antibodies. Related Article: How It Works: A New Approach to Western Blotting That number of citations alone gives anyone an idea of this technique’s value to molecular biology. ![]() In 1979, Harry Towbin of the Friedrich Miescher Institute for Biomedical Research in Basel, Switzerland, and his colleagues Theophil Staehelin and Julian Gordon published an article in the Proceedings of the National Academy of Sciences of the United States called “Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.” This launched a protein-identification technique known as Western blotting, and 54,126 papers have cited Towbin and company’s original publication- according to Google Scholar on September 3, 2016. PDF Version Western Blotting Remains a Go-To Tool That Keeps Getting Easier ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |